The Release of Extracellular Enzymes from Yeast by "Osmotic Shock"

Release of surface enzymes in Enterobacteriaceae by osmotic shock.

The process of osmotic shock, which has been used to release degradative enzymes from Escherichia coli, can be applied successfully to other members of the Enterobacteriaceae. Cyclic phosphodiesterase (3'-nucleotidase), 5'-nucleotidase (diphosphate sugar hydrolase), acid hexose phosphatase, and acid phenyl phosphatase are released from Shigella, Enterobacter, Citrobacter, and Serratia strains. ...

The Release of Enzymes from Kwherichia coli by Osmotic Shock and during the Formation of Spheroplasts

When Escherichia coli cells are converted into spheroplasts by means of ethylenediaminetetraacetate and lysozyme (1)) most of the inducible alkaline phosphatase (2, 3) is released into the surrounding sucrose-tris(hydroxymethyl)aminomethane-HCl medium (4, 5), and a large fraction of the “latent” ribonuclease (6-8) and the ribonucleic acid inhibited deoxyribonuclease (9) is also set free (lO).r ...

The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts.

When Escherichia coli cells are converted into spheroplasts by means of ethylenediaminetetraacetate and lysozyme (1)) most of the inducible alkaline phosphatase (2, 3) is released into the surrounding sucrose-tris(hydroxymethyl)aminomethane-HCl medium (4, 5), and a large fraction of the “latent” ribonuclease (6-8) and the ribonucleic acid inhibited deoxyribonuclease (9) is also set free (lO).r ...

The release of enzymes by osmotic shock from Escherichia coli in exponential phase.

Release of extracellular enzymes from Bacillus amyloliquefaciens.

Washed-cell suspensions of Bacillus amyloliquefaciens secrete significant amounts of the extracellular enzymes alpha-amylase and protease for about 15 min in the almost complete absence of protein synthesis. This apparently represents release of preformed enzyme en route to secretion. The release was independent of energy but was affected by temperature. Pulse-labeling experiments showed that n...